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In this study, we established UUO mice, which exhibited severe tubulointerstitial injury (such as brush border loss, interstitial oedema, renal tubular dilation and tubular epithelial cell necrosis/loss), as reported by others and our group 19,29

Injecting twice or three times daily, as some protocols require, becomes burdensome

Future studies that will use more SIRT1 activators, such as metformin (71, 72) and SRT1720 (73), are warranted in order to elucidate further the interplay among FAO, altered NAD + /NADH ratio, SIRT1, SIRT3, and acetyltransferases, such as GCN5 (74), in regulating acetylation and activation of PGC1 along with acetylation of mitochondrial proteins

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